SMART Digest compared to classic in-solution digestion of rituximab for in-depth peptide mapping characterization
نویسنده
چکیده
Introduction Peptide mapping is a common technique in the biopharmaceutical industry to characterize monoclonal antibodies (mAbs) for the determination of product identity and stability. Many conventional sample preparation methods are time consuming with digestion times of several hours and can introduce modifications such as deamidation, oxidation, and carbamylation.1 In this study, two classic in-solution digestion approaches were compared to the recently developed SMART Digest kit method to quantify the extent of posttranslational and chemical modifications of a therapeutic recombinant mAb. The critical requirements for each Authors: Martin Samonig1, Alexander Schwahn2, Ken Cook3, Mike Oliver4, and Remco Swart1 1Thermo Fisher Scientific, Germering, Germany; 2Thermo Fisher Scientific, Basel, Switzerland; 3Thermo Fisher Scientific, Hemel Hempstead, United Kingdom; 4Thermo Fisher Scientific, Runcorn, United Kingdom
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